Anti-Acetylcholinesterase Monoclonal Antibody from ABR - AFFINITY BIOREAGENTS
| Antigenic Specificity | Acetylcholinesterase |
|---|---|
| Clone | 400000000000 |
| Host Species | Mouse |
| Reactive Species | cow, human |
| Isotype | IgG1 |
| Format | 200 ug of Protein A/G purified antibody (1 mg/ml) in 0.01 M phosphate buffer with 0.5 M NaCl and 15 mM sodium azide; pH 7.4. |
| Size | 200 ug, 1 mg/ml |
| Applications | WB, ELISA |
| Description | HYB 190-01-02 is specific for brain AChE and does not recognize AChE from erythrocytes. The antibody can thus distinguish between mammalian brain AChE and eruthrocyte AChE. Weak cross-reactivity with Torpedo marmorata AChE but none with AChE from electric eel or human BtChE.HYB 190-01-02 has been successfully used in ELISA and Western blotting techniques. Can be used in ELISA on amniotic fluid for the diagnosis of neural tube defects. HYB 190-01-02 is well suited as catching antibody (on an anti-mouse IgG coat) in enzyme antigen immunoassay (EAIA), where the antigen (AChE) is captured and used directly as substrate for acetylthiocholiniodide (Ellmann's reaction). In Western blotting and dot blotting HYB 190-01-02 reacts with native and denatured human and bovine, detergent soluble and salt-soluble AChE. No cross-reactivity is seen with erythrocyte AChE.The HYB 190-01-02 immunogen is the C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the eruthrocyte enzyme. Coupled to PPD with glutaraldehyde.The epitope of HYB 190-01-02 is C-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (DS-AChE and SS-AChE). |
| Catalog # | HYB 190-01-02 |
| Price | $475.87 |
| Other Names | n/a |
| Supplier Data Page | Anti-Acetylcholinesterase from ABR - AFFINITY BIOREAGENTS |
ABR - AFFINITY BIOREAGENTS
now sold as Thermo Scientific
4620 Technology Dr. #600
Golden CO 80403
Phone: 303-278-4535
Phone: 800-527-4535
Fax: 303-278-2424
Email: affinity@bioreagents.com
Website: http://www.thermo.com/abr
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