Adenovirus from UNITED STATES BIOLOGICAL

Name Adenovirus
Description Applications:Detection of antibodies to adenovirus in ELISA. Use in concentration 2.5-5mg/ml.Morphology: In investigation using electron microscopy typical adenovirus particles 80nm in diameter were observed.Source:Tonsil strain of AV was grown on monolayer of continuous lines of HeLa.Inactivation:Infectivity of the virus was destroyed by thimerosal (final concentration 1:5000).Purification:When the cultures showed pronounced c.p.e., virus-containing medium was purified from cellfragments by low speed centrifugation (4000 rpm 40 min). Virus was pelleted by high speedcentrifugation at 18000 rpm for 2.5h in the SW27 rotor. The virus pellet was collected, diluted Supplied in 6ml STE buffer and layered over linear 30 to 60% sucrose gradient prepared in STE buffer. Sample recentrifuged at 25000 rpm in the SW27 rotor for 2.5h at 4?C, then the virus band was collected, diluted with STE buffer and pelleted by centrifugation at 30000 rpm for 1.5h at 4?C. The pellet of virus was collected and diluted with STE buffer.Storage & Stability:Store at -70?C about 6 months.
Size 500ug
Applications ELISA
Catalog # A0876-35B
Price n/a
Other Names n/a
Supplier Data Page Adenovirus from UNITED STATES BIOLOGICAL

UNITED STATES BIOLOGICAL

P.O. Box 261

Swampscott MA 01907

Phone: 781-639-5092

Phone: 800-520-3011

Fax: 781-639-1768

Email: chemicals@usbio.net

Website: http://www.usbio.net

 
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