b-Amyloid, aa1-42 from UNITED STATES BIOLOGICAL

Name b-Amyloid, aa1-42
Description Major constituent of plaques and tangles that occur in Alzheimer's disease (AD) patientsSequence: H2N-Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe- Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile- Ala-OHAmino Acid Analysis and Identity:Confirms expected sequencePeptide Content: 73.89 + 4%Supplied As: Trifluoroacetate salt; 1.0 mg net peptide/vialPhysical Appearance: White, lyophilized powderSolubility: H2O, DMSO, HFIP (1,1,1,3,3,3-hexafluoro-2-propanol). Aqueous 5% NH4OH Storage: -20?CRecommendations for Peptide Reconstitution:Preparing peptide for neurotoxicity studies, to induce peptide aggregation:The peptide is supplied in a form that is not neurotoxic prior to a preincubation step. The appearance of toxicity has been shown to correlate with the extent of beta sheet structure (Simmons, L.K. et al. [1994] Mol. Pharmacology 45(3):373-379).Recommended preincubation procedure:1. Dissolve the lyophilized peptide in HPLC grade (or better) water at E 6 mg/mL.2. Dilute the peptide to 1 mg/mL with PBS (it is important that Ca2+ is omitted).3. Incubate at 37?C for 24-48 h (24-36 h is usually sufficient). Neurotoxicity is usually observed at 30-100ug/ml. Preparing peptide for studies which require minimal peptide aggregation:1. Dissolve the peptide at a concentration of 1 mg/mL in 100% HFIP (1,1,1,3,3,3-hexafluoro-2-propanol [Sigma-Aldrich, 99.8% ACS reagent grade]).2. Incubate at RT for 1 hour with occasional vortexing at moderate speed.3. Sonicate for 10 minutes in a water bath sonicator while the tube is held in the upright position.4. Dry down the HFIP/peptide solution under a gentle stream of nitrogen gas. Continue drying for an additional 10 minutes. Cap vial immediately.5. Resuspend the peptide in 100% DMSO.6. Incubate the peptide plus DMSO for 12 minutes at RT with periodic vortexing at moderate speed. The vial should be rotated during this incubation to permit thorough wetting of the peptide.7. Add 10ul of this DMSO/peptide solution to 10ml of BSAT-DPBS (see formulation below).8. Vortex the solution at moderate speed. Peptides prepared in this manner have been used as standards in ELISA for the detection of beta amyloid in biological samples. When using this peptide in ELISA, it is important that the assay buffer used with the peptide standards has the same composition as the samples under investigation.Buffer Formulations:DPBS Solution (10X Stock)Dulbecco's PBS (DPBS w/o Mg2+, Ca2+)BSAT-DPBS Solution:1X DPBS, pH 7.45% BSA0.03% Tween-20Note: The inclusion of a protease inhibitor is recommended when the BSAT-BPBS solution is to be used for the analysis beta amyloid-containing biological samples. To prepare a 40 mM stock solution of AEBSF, add 100 mg of AEBSF to 10ml DPBS, pH 7.4, containing 5% BSA. The stock solution should be diluted to a concentration of 1 mM AEBSF in BSAT-DPBS just prior to use.
Size 1mg
Applications n/a
Catalog # A2275-77S
Price please inquire
Other Names n/a
Supplier Data Page b-Amyloid, aa1-42 from UNITED STATES BIOLOGICAL

UNITED STATES BIOLOGICAL

P.O. Box 261

Swampscott MA 01907

Phone: 781-639-5092

Phone: 800-520-3011

Fax: 781-639-1768

Email: chemicals@usbio.net

Website: http://www.usbio.net

 
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