TGR5 (GP-BAR1) Reporter Assay Kit from CAYMAN CHEMICAL COMPANY
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| Product Name | TGR5 (GP-BAR1) Reporter Assay Kit |
| Description | Cayman's TGR5 (GP-BAR1) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for expressing TGR5 and a cAMP response element regulated secreted alkaline phosphatase (SEAP) reporter (TGR5 Reverse Transfection Strip Plate). Cells grown on the transfection complex will express TGR5 at the cell surface within 24 hours. Binding of agonists to TGR5 initiates a signal transduction cascade through the GαS and adenylate cyclase pathway resulting in the expression of SEAP which is secreted into the cell culture medium. Aliquots of culture medium are collected at 6-8 hours after stimulation, and SEAP activity is measured following the addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is easy to use and can be readily applied to high-throughput screening for therapeutic compounds regulating activation of TGR5. A selective synthetic agonist, the TGR5 Receptor Agonist (3-[2-chlorophenyl]-N-[4-chlorophenyl]-N,5-dimethyl-4-isoxazolecarboxamide), is included in the kit for use as a positive control.{27533} The kit provides sufficient reagent to measure SEAP activity at three time points using the three included white assay plates. |
| Size | 1 ea |
| Concentration | n/a |
| Applications | Cell-Based Assay, High-Throughput Screening, Reverse Transfection Reporter Assay |
| Other Names | GPBAR GPBAR1 luminescence TGR-5 HEK-293 |
| Gene, Accession, CAS # | n/a |
| Catalog # | 601440-96WELLS |
| Price | $464.00 |
| Order / More Info | TGR5 (GP-BAR1) Reporter Assay Kit from CAYMAN CHEMICAL COMPANY |
| Product Specific References | n/a |
CAYMAN CHEMICAL COMPANY
CAYMAN CHEMICAL COMPANY
1180 East Ellsworth Road
Ann Arbor MI 48108
P: (734) 971-3335
P: (800) 364-9897 (USA & Canada only)
F: (734) 971-3640
Customer Service: custserv@caymanchem.com
Technical Support: techserv@caymanchem.com
Sales: sales@caymanchem.com
http://www.caymanchem.com
Profile of CAYMAN CHEMICAL COMPANY