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AIF1 from UNITED STATES BIOLOGICAL
AIF1 from UNITED STATES BIOLOGICAL
| Name |
AIF1 |
| Description |
Purity: Highly Purified. C-TGPPAKKAISELP, from the C-Terminus of human AIF1.Control peptide corresponding to polyclonal antibody, Catalog#A1059-86L1, goat x human.AIF1 (AIF1, allograft inflammatory factor 1, DASS-82G15.2, AIF-1, IBA1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule) is directed against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognize native protein in any application.This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. The gene expresses three transcripts.Applications:Suitable for use in ELISA. Other Applications not tested.Recommended Dilutions:Optimal dilutions to be determined by the researcherPositive Control:Human Brain (Frontal Cortex) and Rat Brain lysatesStorage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Peptide Blocking (see corresponding antibody A1059-86L1):Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. |
| Size |
100ug |
| Applications |
ELISA |
| Catalog # |
A1059-86L1-P |
| Price |
please inquire |
| Other Names |
AIF1 (Allograft Inflammatory Factor 1, AIF-1, DASS-82G15.2, IBA1, IRT-1, Em:AF129756.17, Interferon gamma Responsive Transcript, Ionized Calcium-binding Adapter Molecule) (Control Peptide) |
| Supplier Data Page |
AIF1 from UNITED STATES BIOLOGICAL |
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