APE from UNITED STATES BIOLOGICAL

UNITED STATES BIOLOGICAL

UNITED STATES BIOLOGICAL

P.O. Box 261

Swampscott MA 01907

Phone: 781-639-5092

Phone: 800-520-3011

Fax: 781-639-1768

Email: chemicals@usbio.net

Website: http://www.usbio.net

View Supplier Profile for UNITED STATES BIOLOGICAL

APE from UNITED STATES BIOLOGICAL

Name	APE
Description	Purity: Highly Purified. PKRGKKGAVAEDGD-C, N TerminusControl peptide corresponding to polyclonal antibody, Catalog#A2298-74N, goat x human.Alternate Name: APEX, APEX1, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APE1, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA-(apurinic or apyrimidinic site) lyase, apurinic/apyrimidinic (abasic) endonuclease, deoxyribonuclease (apurinic or apyrimidinic)Domain Information: Exonuclease III [DNA replication, recombination, and repair]Gene Ontology Terms: DNA-(apurinic or apyrimidinic site) lyase activity; uracil DNA N-glycosylase activity; endodeoxyribonuclease activity; transcription co-repressor activity; 3'-5' exonuclease activity; endonuclease activity; DNA binding; transcription co-activator activity; base-excision repair; DNA repair; transcription from Pol II promoter; obsolete; nucleusApplications: Suitable for use in ELISA. Other applications not tested.Recommended Dilution:Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Peptide Blocking (see corresponding antibody A2298-74N):Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Size	100ug
Applications	ELISA
Catalog #	A2298-74N-P
Price	please inquire
Other Names	APE (APEX, APEX1, APEX nuclease multifunctional DNA repair enzyme, APX, APE1, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, DNA apurinic/apyrimidinic exonuclease/ lyase) (Control Peptide)
Supplier Data Page	APE from UNITED STATES BIOLOGICAL

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