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Linscott's Directory of Immunological & Biological Reagents
UNITED STATES BIOLOGICAL
 (http://www.usbio.net)

UNITED STATES BIOLOGICAL

P.O. Box 261

Swampscott MA 01907

Phone: 781-639-5092

Phone: 800-520-3011

Fax: 781-639-1768

Email: chemicals@usbio.net

Website: http://www.usbio.net

 
ARHU from UNITED STATES BIOLOGICAL

ARHU from UNITED STATES BIOLOGICAL

Name ARHU
Description Purity: Highly Purified. PPQQGDPAFPDRCEA, from the C-Terminus of human ARHU.Control peptide corresponding to polyclonal antibody, Catalog#A3570-67B, goat x human.ARHU is directed against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognize native protein in any application. This protein is a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of Wnt1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation.Applications:Suitable for use in ELISA. Other Applications not tested.Recommended Dilutions:Optimal dilutions to be determined by the researcherStorage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Peptide Blocking (see corresponding antibody A3570-67B):Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Size 100ug
Applications ELISA
Catalog # A3570-67B-P
Price please inquire
Other Names ARHU (CDC42-like GTPase 1, CDC42L1, FLJ10616, hG28K, GTP-binding Protein-like 1, Ryu GTPase, Rho-related GTP-binding Protein RhoU, Rho GTPase-like Protein ARHU, Wnt-1 Responsive Cdc42 Homolog 1, WRCH-1) (Control Peptide)
Supplier Data Page ARHU from UNITED STATES BIOLOGICAL
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