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Antigenic Specificity | TUBULIN ALPHA |
Clone | [YL1/2] |
Host Species | Rat |
Reactive Species | n/a |
Isotype | IgG2a |
Format | unconjugated |
Size | 0.5 mg |
Concentration | IgG concentration 1.0 mg/ml |
Applications | Immunohistology Frozen, ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassays (RIA), Western Blot (WB) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Rat anti Tubulin alpha antibody, clone YL1/2 recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (Wehland et al. 1983). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E Coli rec A and oxidized actin (Burns 1987). This product is r |
Immunogen | Immunogen: Yeast tubulin Fusion Partners: Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line. Buffer Solution: Phosphate buffered saline Target Species: Yeast |
Other Names | n/a |
Gene, Accession # | n/a |
Catalog # | MBS213159 |
Price | $515 |
Order / More Info | TUBULIN ALPHA Antibody from MYBIOSOURCE INC. |
Product Specific References | n/a |