Edit |   |
---|---|
Antigenic Specificity | Phospho-SMAD3 (S208) |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human (predicted reactivity: chicken, mouse, porcine, rat) |
Isotype | n/a |
Format | peptide affinity purified |
Size | 0.08 mL, 0.4 mL |
Concentration | vial concentration: 0.5 |
Applications | Immunofluorescence (IF), Dot Blot (DB), ELISA (EIA) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This SMAD3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S208 of human SMAD3. SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss |
Immunogen | n/a |
Other Names | [Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3] |
Gene, Accession # | [SMAD3], Gene ID: 4088, NCBI: NP_001138574.1, UniProt: P84022 |
Catalog # | MBS9213657 |
Price | $165, $370 |
Order / More Info | Phospho-SMAD3 (S208) Antibody from MYBIOSOURCE INC. |
Product Specific References | References for protein: 1.Imoto, S., et al., FEBS Lett. 579(13):2853-2862 (2005). 2.Dubrovska, A., et al., Oncogene 24(14):2289-2297 (2005). 3.Furumatsu, T., et al., J. Biol. Chem. 280(9):8343-8350 (2005). 4.Kobayashi, T., et al., Biochem. Biophys. Res. Commun. 327(2):393-398 (2005). 5.Kamaraju, A.K., et al., J. Biol. Chem. 280(2):1024-1036 (2005). References for HeLa cell line: 1. Scherer WF, Syverton JT, Gey GO (May 1953). Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. J. Exp. Med. 97 (5): 695-710. [PubMed:13052828]. 2. Macville M, Schrock E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping. Cancer Res. 59 (1): 141-50. [PubMed: 9892199]. 3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). A novel L1 retrotransposon marker for HeLa cell line identification. BioTechniques 46 (4): 277-84. [PubMed: 19450234]. 4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). Check your cultures. A list of cross-contaminated or misidentified cell lines. Int. J. Cancer 127 (1): 1-8. [PubMed:20143388]. |