Biotinylated SARS-CoV-2 S protein (D614G), His, Avitag™, Super stable trimer (MALS verified) from ACROBIOSYSTEMS USA

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Product NameBiotinylated SARS-CoV-2 S protein (D614G), His, Avitag™, Super stable trimer (MALS verified)
DescriptionPurity >95% as determined by SDS-PAGE. Biotinylated SARS-CoV-2 S protein (D614G), His, Avitag, Super stable trimer (SPN-C82E3) is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (D614G)) and D614G mutation, which has become increasingly common in SARS-CoV-2 viruses from around the world. The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. It is the biotinlynated form of SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified) (Cat. No. SPN-C52H3). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 139.7 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Size200ug
Concentrationn/a
Applicationsn/a
Other NamesSpike, S protein, Spike glycoprotein, S glycoprotein, COVID-19
Gene, Accession, CAS #Accession: QHD43416.1 (D614G)
Catalog #SPN-C82E3-200ug
Price$2200
Order / More InfoBiotinylated SARS-CoV-2 S protein (D614G), His, Avitag™, Super stable trimer (MALS verified) from ACROBIOSYSTEMS USA
Product Specific References(1) Wan Y, et al. J Virol. 2020. pii: JVI.00127-20. (2) Benvenuto D, et al. J Med Virol. 2020. (3) Chang CY, et al. AMB Express. 2020. 10(1):20.
ACROBIOSYSTEMS USA
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