beta-Galactosidase from ANIARA

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Product Namebeta-Galactosidase
DescriptionMedicago AB Products Are Available Worldwide For Research Use Only beta-Galactosidase is a hydrolase enzyme that catalyzes the hydrolysis of beta-galactosides into monosaccharides. The protein comprises four chains and has a molecular weight of 464 kDa, each subunit with a weight of 116 kDa. beta-Galactosidase is a commonly used reporter molecule and a very important marker for the lacZ gene. The enzyme can be split into two peptides; LacZalpha and LacZomega. Neither is active by itself but both spontaneously reassemble into a functional enzyme. This characteristic is used in many cloning vectors to achieve a-complementation in specific laboratory strains of E. coli (the small LacZalpha peptide is encoded by the plasmid while the large LacZomega is encoded in trans by the bacterial chromosome). When DNA fragments are inserted in the vector and production of LacZalpha disrupted, cells exhibit no beta-galactosidase activity. This allows the blue/white screening of recombinant clones. Specific activity is 230-375 Units/mg protein at 410 nm, pH 7.0 and 25 degrees Celsius (0.2 M Na-phosphate, 2 mM MgCl2, 8% methanol, 0.25% Tween 20). One unit of enzyme hydrolyses 1 micromole of ortho-nitro-phenyl-beta-D-Galactopyranoside (ONPG) (46 mM) per minute. The protein concentration is assayed by the biuret method with BSA as standard. The free thiol groups on the enzyme surface are characterized to be 14-18 mole/mole enzyme. These free groups are useful for derivatisation of the enzyme. One vial contains approximately 25 mg (8 KU) lyophilizate of partly purified enzyme. Reconstitution of this lyophilizate in 4 ml of water yields a solution of approximately 1 mM Tris-HCI, pH 7.2 and 1 mM MgCl2. For laboratory use only.
Size25 mg
Other Namesn/a
Gene, Accession, CAS #n/a
Catalog #A10-1101-25
Order / More Infobeta-Galactosidase from ANIARA
Product Specific Referencesn/a
7768 Service Center Dr.
West Chester OH 45069
P: 513-770-1991
P: 866-783-3797
F: 513-573-9241

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