Human SARS-CoV-2 Spike Protein S2 Antigen ELISA (Quantitative) from EAGLE BIOSCIENCES INC.

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Product NameHuman SARS-CoV-2 Spike Protein S2 Antigen ELISA (Quantitative)
DescriptionThe SARS-CoV-2 (Covid-19) Spike Protein S2 Antigen ELISA Assay Kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit. The spike protein (S-protein) of the SARS-CoV-2 virus is the common target for neutralizing antibodies and vaccines. Spike protein contains two subunits, S1 and S2. S1 contains a receptor binding domain (RBD), which is responsible for recognizing and binding with the cell surface receptor. S2 subunit contains other basic elements needed for the membrane fusion. SARS-CoV-2 (2019-nCoV) (ref.) can infect the human respiratory epithelial cells through interaction with huamn ACE2. Indeed, the recombinant Spike protein can bind with recombinant ACE2 protein. Sample types: serum/plasma/cell culture supernatant. Range: 0-500 ng/ml. Sensitivity: 6.8 ng/ml. Time: 3 hrs
Size12 x 8 wells
ApplicationsThe method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human SARS-CoV-2 (Covid-19) spike protein S2 present in the sample are bound by the capture antibodies. After incubation the wells are washed and followed by HRP- conjugated Detection Antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-CoV-2 (Covid-19) spike proteins in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Other Namesn/a
Gene, Accession, CAS #n/a
Catalog #KBVH015-18
Order / More InfoHuman SARS-CoV-2 Spike Protein S2 Antigen ELISA (Quantitative) from EAGLE BIOSCIENCES INC.
Product Specific Referencesn/a
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110

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