ADMA Xpress ELISA Kit from IMMUNDIAGNOSTIK AG
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| Product Name | ADMA Xpress ELISA Kit |
| Description | This assay is based on the method of competitive enzyme linked immunoassays. The sample preparation includes the addition of a derivatization reagent for ADMA derivatization, and a reaction buffer is added containing ADMA-derivative (tracer). Afterwards, the treated samples are incubated in wells of a microtiter plate coated with a polyclonal antibody against ADMA-derivative. During the incubation period the target ADMA in the sample competes with the tracer for the binding of the polyclonal antibodies immobilized on the wall of the microtiter wells. The ADMA in the sample displaces the tracer out of the binding to the antibodies. Therefore, the concentration of the antibody-bound tracer is inverse proportional to the ADMA concentration in the sample. During the second incubation step a peroxidase conjugate is added to each microtiter well to detect the tracer. After washing away the unbound components tetramethylbenzidine (TMB) is added as a peroxidase substrate. Finally, the enzymatic reaction is terminated by an acidic stop solution. The color changes from blue to yellow, and the absorbance is measured in a photometer at 450 nm. The intensity of the yellow color is inverse proportional to the ADMA concentration in the sample; this means, high ADMA concentration in the sample reduces the concentration of antibody-bound tracer and lowers the photometric signal. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. ADMA present in the patient samples is determined directly from this curve. Range: 0,1-2,0 µmol/l |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | ADMA Xpress |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 7860 |
| Price | please inquire |
| Order / More Info | ADMA Xpress ELISA Kit from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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