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Antigenic Specificity | CD85j (ILT2), Human |
Clone | REA998 |
Host Species | Recombinant Human |
Reactive Species | human |
Isotype | IgG1 |
Format | fluorescein (FITC) conjugate |
Size | 100 tests in 200 µL |
Concentration | 1:50 |
Applications | Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | CD85j (ILT2) Antibody, anti-human, FITC, REAfinity™. Clone REA998 recognizes the human CD85j (ILT2) antigen, an inhibitory immunoreceptor of the immunoglobulin-like transcript (ILT) family. CD85j is a type I transmembrane protein with four extracellular Ig-like domains and four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic tail. The inhibitory signal is transmitted by recruiting SH2-containing protein tyrosine phosphatase-1 that dephosphorylates and inactivates downstream tyrosine kinases. Expression of CD85j is found on B cells, monocytes, dendritic cells, and subsets of natural killer (NK) and T cells. CD85j is known to bind classical and non-classical MHC class I molecules, human cytomegalovirus UL18 protein, and some HLA-C alleles, as ligands. Crosslinking of CD85j prevents activation of B cells, T cells, NK cells, macrophages, and downregulation of effector mechanisms, such as NK and T cells cytolytic functions. | Additional information: Clone REA998 displays negligible binding to Fc receptors. |
Immunogen | n/a |
Other Names | LILRB1, ILT2, LIR-1, MIR-7 |
Gene, Accession # | Gene ID: 10859 |
Catalog # | 130-116-614 |
Price | $270 |
Order / More Info | CD85j (ILT2), Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | Borges, L. et al. (1997) A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules. J Immunol 159: 5192-5196. | Morel, E. and Bellon, T. (2008) HLA Class I molecules regulate IFN-{gamma} production induced in NK cells by target cells, viral products, or immature dendritic cells through the inhibitory receptor ILT2/CD85j. J Immunol 181: 2368 -2381. | Merlo, A. et al. (2005) Inhibitory receptors CD85j, LAIR-1, and CD152 down-regulate immunoglobulin and cytokine production by human B lymphocytes. Clin. Diagn. Lab. Immunol. 12: 705-712. |