Description | Specificity: HHF35 reacts with both alpha-muscle and gamma-smooth muscle actin, and therefore reacts with skeletal muscle, cardiac muscle, vascular and visceral smooth muscle cells, pericytes and myoepithelial cells. It is also reactive in myofibroblasts. It does not react with epithelial, endothelial, neural or normal connective tissue cells when applied under the proper conditions to these tissue sections. Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These act |
Product Specific References | Tsukada, T., Tippens, D., Gordon, D., Ross, R. and Gown, A.M. (1987). HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. American Journal of Pathology 126, 51-60.Tsukada, T., McNutt, M.A., Ross, R. and Gown, A.M. (1987). HHF35, a muscle-actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic Human tissues. American Journal of Pathology 127, 389-402.Schmidt, R.A., Cone, R., Haas, J.E. and Gown, A.M. (1988). Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed anti muscle actins. American Journal of Pathology 131, 19-28.Babaev, V.R., Bobryshev, Y.V., Stenina, O.V., Tararak, E.M. and Gabani, G. (1990). Heterogeneity of smooth muscle in atheromatous plaque of Human aorta. American Journal of Pathlogy 136, 1031-42.Nascimento, C., Caroli-Bottino, A., Paschoal, J. and Pannain, V.L. (2009). Vascular immunohistochemical markers: contributions to hepatocellular nodule diagnosis in explanted livers. Transplantation Proceedings 41, 4211-13. |