MojoSort™ Mouse F4/80 Selection Kit from BIOLEGEND

Mouse F4/80+ cells are either selected or depleted by incubating the sample with the Biotin anti-mouse F4/80 Antibody Cocktail followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The magnetically labeled fraction contains the cells of interest; do not discard this fraction. After collection of the F4/80+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. 

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage. Please contact our technical service team for further assistance.