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Product Name | SARS-CoV-2 Surrogate Virus Neutralization Test ELISA |
Description | The method employs sandwich ELISA technique. The protein-protein interaction between HRP-RBD and hACE2 can be blocked by neutralizing antibodies against SARS-CoV-2 RBD. Samples and controls are pipetted in a blank microtitre plate and incubated with HRP conjugated human SARSCoV-2 RBD protein. The antibodies to SARS-CoV-2 present in the samples and controls bind to the SARSCoV-2 RBD protein to form a complex. This solution of bound and unbound antibodies to SARS-CoV-2 is then pipetted into human ACE2 coated microplate. After washing to remove the bound complex of Anti-SARS-CoV-2 and HRP conjugated SARSCoV-2 RBD, the substrate solution (TMB) is added to the microwells. Post incubation, color develops proportionally to the amount of unbound Anti-SARS-CoV-2 (Covid-19) present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm. Sample types: serum, plasma. Incubation time: 3 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | KBVH400 |
Price | $790 |
Order / More Info | SARS-CoV-2 Surrogate Virus Neutralization Test ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |