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Product Name | Mouse Serum Amyloid P (SAP) ELISA |
Description | The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the SAP present in samples reacts with the anti-SAP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAP in the test sample. The quantity of SAP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Sample types: serum, plasma. Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.845 ng/ml. Incubation time: 130 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Serum Amyloid P (SAP) ELISA test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | MSP11-K01 |
Price | $635 |
Order / More Info | Mouse Serum Amyloid P (SAP) ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |