Edit |   |
---|---|
Product Name | Mouse GDF-15 ELISA Kit (Replaced with Toronto Biosciences, SAME CAT#) |
Description | This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a polyclonal antibody specific for mouse GDF-15. Standards and samples are pipetted into the wells and any mouse GDF-15 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for mouse GDF-15 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse GDF-15 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse GDF-15, the unknown sample concentration can be interpolated from a reference curve included in each assay. Sample types: serum/plasma/cell culture supernatant. Range: 3.9-250 pg/ml. Sensitivity: 3.9 pg/ml. Incubation time: 3 hr 35 min |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | GDF-15, Growth differentiation factor 16 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | MHF11-K01 |
Price | $685 |
Order / More Info | Mouse GDF-15 ELISA Kit (Replaced with Toronto Biosciences, SAME CAT#) from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |