High Sensitive Human Fetuin-A ELISA Assay Kit from EAGLE BIOSCIENCES INC.
Search, find, compare suppliers for High Sensitive Human Fetuin-A ELISA Assay Kit products.
| Edit | |
|---|---|
| Product Name | High Sensitive Human Fetuin-A ELISA Assay Kit |
| Description | This Eagle Biosciences High Sensitive Human Fetuin-A ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human Fetuin-A in serum samples. The assay utilizes the two-site ``sandwich'' technique with two selected goat anti-human Fetuin-A polyclonal antibodies that bind to different epitopes of human Fetuin-A. Assay standards, controls and prediluted patient serum samples containing human Fetuin-A are added to microtiter wells of a microplate that was coated with a high affinity polyclonal goat anti-human Fetuin-A antibody. After the first incubation period, the antibody on the wall of microtiter well captures human Fetuin-A in the sample and unbound proteins in each microtiter well are washed away. Then a horseradish peroxidase (HRP)-conjugated polyclonal anti-human Fetuin-A antibody is added to each microtiter well and a ``sandwich'' of ``capture antibody - human Fetuin-A - HRP-conjugated tracer antibody'' is formed. The unbound tracer antibody is removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to the Fetuin-A on the wall of the microtiter well is directly proportional to the amount of Fetuin-A in the sample. A standard curve is generated by plotting the absorbance versus the respective human Fetuin-A concentration for each standard on point-to-point or cubical scales. The concentration of human Fetuin-A in test samples is determined directly from this standard curve. Sample types: serum. Range: 0 -4343 ng/mL. Sensitivity: 40 ng/mL. Incubation time: 3 hours |
| Size | 1 x 96 well |
| Concentration | n/a |
| Applications | RUO |
| Other Names | Human Fetuin-A, sensitive test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | HFA31-K01 |
| Price | $1105 |
| Order / More Info | High Sensitive Human Fetuin-A ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.