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Product Name | Mouse TGF beta1 ELISA Kit |
Description | This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for TGF beta1 has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any TGF beta1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for TGF beta1 is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of TGF beta1 bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm.The concentration of TGF beta1 in the sample is then determined by comparing the O.D of samples to the standard curve. Sample types: serum, plasma, cell culture supernatants. Range: 31.25 - 2000 pg/ml. Sensitivity: 15 pg/ml. Incubation time: 4 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | TGF beta 1, Transforming Growth Factor test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | ARG80211 |
Price | $715 |
Order / More Info | Mouse TGF beta1 ELISA Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |