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Product Name | Mouse IL-25 (IL-17E) ELISA KIT |
Description | The Eagle Biosciences Mouse Interleukin-25(Interleukin-17E) (IL-25(IL-17E)) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-25(IL-17E) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-25(IL-17E) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-25(IL-17E) is added to the wells and binds to the combination of capture antibody-IL-25(IL-17E) in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-25(IL-17E) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-25(IL-17)E standard dilutions and IL-25(IL-17E) sample concentration determined. Sample types: serum, plasma, cell culture. Range: 31.25 - 1000 pg/ml. Sensitivity: 15 pg/ml. Incubation time: 3.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Interleukin 25(Interleukin 17E), IL-25(IL-17E) test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | L2511-K01 |
Price | $505 |
Order / More Info | Mouse IL-25 (IL-17E) ELISA KIT from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |