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Product Name | Mouse Fetuin ELISA |
Description | The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Fetuin A present in samples reacts with the anti-Fetuin A antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-FET-A antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound FET-A. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo-genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of FET-A in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of FET-A in the test sample. The quantity of FET-A in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Sample types: biological fluids. Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 0.846 ng/ml. Incubation time: 50 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Fetuin test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | MFN19-K01 |
Price | $600 |
Order / More Info | Mouse Fetuin ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |