Haptogloblin Typing ELISA Assay Kit from EAGLE BIOSCIENCES INC.

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Product NameHaptogloblin Typing ELISA Assay Kit
DescriptionHaptoglobin (Hp) typing ELISA pates are supplied coated with purified monoclonal antibody (mAb) directed against Hp. The serum to be tested in diluted and incubated in the Hp ELISA plate. In this step Hp in the serum binds to the immobilized antibody. The non-bound Hp is removed by washing. Horseradish Peroxidase (HRP) conjugated monoclonal antibody to Hp is added. Note: the same mAb in the unconjugated form was used to coat the microtiter wells. Since Hp 1-1 is dimeric, at most only one HRP conjugated mAb can bind per dimer attached to the well. However, Hp 2-1 and Hp 2-2 are polymeric and can potentially nind 2-8 HRP conjugated mAbs. Unbound conjugate is removed by washing. TMB substrate reagent is added resulting in development of a blue color. The blue color development is stopped with the addition of stop solution changing the color to yellow. Absorbance is measured using a spectrophotometer at 450 nm. The absorbance of each sample is compared to a user-calculated cut-off to determine its Hp phenotype. Sample types: diabetic serum/plasma. Sensitivity: Cut-off. Incubation time: 1.5 hours
Size1 x 96 well
Concentrationn/a
ApplicationsRUO
Other NamesHaptogloblin, Hp, Diabetes test, kit, assay, EIA, enzyme immunoassay
Gene, Accession, CAS #n/a
Catalog #710-01
Price$655
Order / More InfoHaptogloblin Typing ELISA Assay Kit from EAGLE BIOSCIENCES INC.
Product Specific Referencesn/a
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110

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