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Product Name | 21-Hydroxylase (21-OH Ab) ELISA |
Description | The assay utilizes the ability of 21-OH Abs to act divalently, and to form a bridge between immobilized 21-OH and 21-OH-Biotin in the fluid phase. In the first step, 21-OH Ab present in samples binds with 21-OH immobilized onto the microtiter plate. In the second step, 21-OH-Biotin binds to this complex. The amount of 21-OH-Biotin bound correlates with the level of antibodies present in patient samples. Unbound 21-OH-Biotin is then removed by washing. Bound 21-OH-Biotin can then be quantified by addition of streptavidin peroxidase (SA-POD) and a colorigenic substrate (TMB), and reading the optical density at 450nm. Sample types: serum. Range: 0.01 - 100 U/ml. Sensitivity: 0.13 U/ml. Incubation time: overnight + 100 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | 3789 |
Price | $1875 |
Order / More Info | 21-Hydroxylase (21-OH Ab) ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |