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Product Name | Mouse Myoglobin ELISA |
Description | The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the MYO present in samples reacts with the anti-MYO antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-MYO antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound MYO. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of MYO in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of MYO in the test sample. The quantity of MYO in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Sample types: serum, plasma. Range: 9.375 ng/ml - 300 ng/ml. Sensitivity: 5.156 ng/ml. Incubation time: 100 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Myoglobin test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | MMY11-K01 |
Price | $580 |
Order / More Info | Mouse Myoglobin ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |