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Product Name | Fecal Rotavirus Antigens ELISA Assay Kit Assay Kit |
Description | This Eagle Biosciences Fecal Rotavirus Antigens ELISA is designed, developed and produced for the qualitative and quantitative measurement of rotavirus Antigens in test specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter well. Assay controls and fecal specimen, as well as HRP-conjugated monoclonal antibody that specifically recognize the inner capsid protein of the rotaviruses are added to microtiter wells of microplate that was coated with a highly purified polyclonal anti-rotavirus antibody on its wall. After an incubation period an immunocomplex of ``Anti-Rotavirus Antibody - Rotavirus Antigens - HRP-conjugated Anti-rotavirus Tracer Antibody'' was formed if there is rotavirus Antigens present in the test sample. The unbound tracer antibody and other protein or buffer matrix are removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to rotavirus captured on the wall of each microtiter well is directly proportional to the amount of rotavirus Antigens level in each test specimen. Sample types: stool. Range: Cut-off. Sensitivity: Cut-off. Incubation time: 2 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Rotavirus, Fecal, VP6 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | RTV35-K01 |
Price | $645 |
Order / More Info | Fecal Rotavirus Antigens ELISA Assay Kit Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |