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Product Name | Mouse / Rat Glucagon ELISA |
Description | The Mouse/Rat Glucagon ELISA Assay Kit is a solid phase two-site enzyme immunoassay. It is based on the direct sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the glucagon molecule. During incubation glucagon in the sample reacts with peroxidase-conjugated antiglucagon antibodies (clone E6A11K) and anti-glucagon antibodies (clone M5F9S) bound to microplate wells. A simple washing step removes unbound enzyme labelled antibody. The bound conjugate is detected by reaction with 3,3',5,5'-tetramethylbenzidine (TMB). The reaction is stopped by adding acid to give a colorimetric endpoint that is read spectrophotometrically. Sample types: serum, EDTA plasma, cell-culture medium. Range: 2 - 180 pmol/L (7 - 627 pm/mL). Sensitivity: 1.5 pmol/L. Incubation time: overnight |
Size | 12 x 8 wells |
Concentration | n/a |
Applications | RUO |
Other Names | Glucagon test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | 10-1281-01 |
Price | $835 |
Order / More Info | Mouse / Rat Glucagon ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |