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Product Name | Mouse IL-33 ELISA |
Description | This assay is a quantitative sandwich ELISA. The microplate is pre-coated with a polyclonal antibody specific for mouse IL-33. Standards and samples are pipetted into the wells and any mouse IL-33 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for mouse IL-33 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse IL-33 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse IL-33, the unknown sample concentration can be interpolated from a reference curve included in each assay. Sample types: serum/plasma/cell culture supernate. Range: 31.25-2000 pg/ml. Sensitivity: 6.5 pg/ml. Incubation time: 3 hrs 35 mins |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | IL-33, Interleukin 3 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | ILT11-K01 |
Price | $695 |
Order / More Info | Mouse IL-33 ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |