Mouse / Rat 25-OH Vitamin D ELISA from EAGLE BIOSCIENCES INC.
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| Product Name | Mouse / Rat 25-OH Vitamin D ELISA |
| Description | Assay calibrators, controls and test samples are added directly to wells of a microtiter plate that is coated with specific anti-25-OH Vitamin D2, D3 antibody. A buffer designed to release Vitamin D from binding proteins is then added to the wells. After the first incubation period, unbound material is washed away and biotinylated Vitamin D analogue is added to the wells and binds to remaining antibody sites. After the second incubation period, unbound biotin-D is washed away and horseradish peroxidase (HRP) conjugated streptavidin is added to each well. During the third incubation step, an immune complex of well coated ``vitamin D antibody - vitamin D, biotin D and HRP conjugated streptavidin'' is formed. The unbound matrix is removed in the subsequent washing steps. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction, which is terminated with an acidic reagent (ELISA stop solution). The absorbance is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is inversely proportional to the amount of total 25-OH Vitamin D2/3 in the test sample. A calibration curve is generated by plotting the absorbance versus the respective Vitamin D concentration for each calibrator on a 4-parameter or point to point curve fitting. The concentration of total 25-OH Vitamin D2/3 in test samples is determined directly from this calibration curve. Sample types: serum, plasma. Range: 9 - 150 ng/mL. Sensitivity: 0.95 ng/mL. Incubation time: 2 hours |
| Size | 1 x 96 well |
| Concentration | n/a |
| Applications | RUO |
| Other Names | Vitamin D, 25-OH, 25 hydroxy, Cholecalciferol, calcidiol, calcitriol test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | VID21-K01 |
| Price | $520 |
| Order / More Info | Mouse / Rat 25-OH Vitamin D ELISA from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.