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Product Name | Hamster (CHO) Nidogen-1 ELISA Assay Kit |
Description | The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Nidogen-1 (NDN) present in samples reacts with the anti-NDN antibodies which have been absorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-NDN is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added, and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of NDN in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of NDN in the test sample. The quantity of NDN in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution. Sample types: tissue culture fluids. Range: 0.3125 ng/mL - 20 ng/mL. Incubation time: 2 hours 50 minutes |
Size | 96 wells |
Concentration | n/a |
Applications | RUO |
Other Names | Nidogen-1 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | NDG91-K01 |
Price | $1440 |
Order / More Info | Hamster (CHO) Nidogen-1 ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |