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Product Name | Mouse sRANK Ligand / TNFSF11 / TRANCE ELISA Kit |
Description | The Eagle Biosciences Mouse sRANK Ligand ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for sRANK Ligand / TNFSF11 / TRANCE has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any sRANK Ligand / TNFSF11 / TRANCE present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for sRANK Ligand / TNFSF11 / TRANCE is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of sRANK Ligand / TNFSF11 / TRANCE bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of sRANK Ligand / TNFSF11 / TRANCE in the sample is then determined by comparing the O.D of samples to the standard curve. Sample types: serum, plasma, cell culture supernatants. Range: 31.25 - 2000 pg/ml. Sensitivity: 15 pg/ml. Incubation time: 4 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | sRANK Ligand, TNFSF11, TRANCE test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | ARG80222 |
Price | $2065 |
Order / More Info | Mouse sRANK Ligand / TNFSF11 / TRANCE ELISA Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |