Retinal Cell Line (R28) from KERAFAST, INC.

R28-immortalized cell characterization

1. Seigel, GM, 1996. Establishment of an E1A-immortalized rat retinal cell culture, In Vitro Cell. Devel. Biol. 32: 66-68.
2. Seigel, GM, Mutchler, A.L., and E.L. Imperato.  1996. Expression of glial cell markers in a retinal precursor cell line. Mol. Vis. 2: 2.
3. Seigel, GM, Takahashi, M., Adamus, G., McDaniel, T. 1998.  Intraocular transplantation of E1A-immortalized retinal precursor cells. Cell Transplant. 7 (6):  559-566.
4. Seigel, GM, Sun, W, Wang, J., Hershberger, DH, Campbell, LM, Salvi, RJ. 2004. Neuronal gene expression and function in the growth-stimulated R28 retinal precursor cell line.  Curr. Eye Res., 28 (4):257-269.
5. Seigel, GM and Salvi, RJ. A microarray dataset of genes expressed by the R28 retinal precursor cell line. Dataset Papers in Neuroscience, 2013, ID: 261063. View Article
6. Seigel GM. R28 retinal precursor cells: The first 20 years. Mol Vis. 2014; 20: 301-306. View Article

R28-immortalized cell utilization

1. Uddin MI, Evans SM, Craft JR, Capozzi ME, McCollum GW, Yang R, Marnett LJ,Uddin MJ, Jayagopal A, Penn JS. In Vivo Imaging of Retinal Hypoxia in a Model of Oxygen-Induced Retinopathy. Sci Rep. 2016 Aug 5;6:31011. View Article
2. Su PJ, Liu Z, Zhang K, Han X, Saito Y, Xia X, Yokoi K, Shen H, Qin L. Retinal synaptic regeneration via microfluidic guiding channels. Sci Rep. 2015 Aug28;5:13591. doi: 10.1038/srep13591. View Article
3. Song Y, Hong S, Iizuka Y, Kim CY, Seong GJ. The neuroprotective effect of maltol against oxidative stress on rat retinal neuronal cells. Korean J Ophthalmol. 2015 Feb;29(1):58-65. doi: 10.3341/kjo.2015.29.1.58. View Article
4. Identification of pigment epithelium-derived factor protein forms with distinct activities on tumor cell lines. Subramanian P, Deshpande M, Locatelli-Hoops S, Moghaddam-Taaheri S, Gutierrez D, Fitzgerald DP, Guerrier S, Rapp M, Notario V, Becerra SP. J Biomed Biotechnol. 2012;2012:425907. Epub 2012 Jun 4.
5. Mouse acetylcholinesterase enhances neurite outgrowth of rat R28 cells through interaction with laminin-1.  Sperling LE, Klaczinski J, Schütz C, Rudolph L, Layer PG. PLoS One. 2012;7(5):e36683. Epub 2012 May 3.
6. Blue light stress in retinal neuronal (R28) cells is dependent on wavelength range and irradiance.  Knels L, Valtink M, Roehlecke C, Lupp A, de la Vega J, Mehner M, Funk RH. Eur J Neurosci. 2011 Aug;34(4):548-58. doi: 10.1111/j.1460-9568.2011.07790.x. Epub 2011 Jul 22.
7. Inhibition of reactive gliosis prevents neovascular growth in the mouse model of oxygen-induced retinopathy. DeNiro M, Al-Mohanna FH, Al-Mohanna FA.  PLoS One. 2011;6(7):e22244. Epub 2011 Jul 14.
8. The effects of commercially available preservative-free FDA-approved triamcinolone (Triesence®) on retinal cells in culture. Zacharias LC, Estrago-Franco MF, Ramirez C, Kenney MC, Takahashi WY, Seigel GM, Kuppermann BD.  J Ocul Pharmacol Ther. 2011 Apr;27(2):143-50.
9. Boriushkin E, Wang JJ, Li J, Jing G, Seigel GM, Zhang SX. Identification of p58IPK as a novel neuroprotective factor for retinal neurons. Invest Ophthalmol Vis Sci. 2015 Feb 5;56(2):1374-86. View Article
10. Chan JF, Yip CC, Tsang JO, Tee KM, Cai JP, Chik KK, Zhu Z, Chan CC, Choi GK, Sridhar S, Zhang AJ, Lu G, Chiu K, Lo AC, Tsao SW, Kok KH, Jin DY, Chan KH, Yuen KY. Differential cell line susceptibility to the emerging Zika virus: implications for disease pathogenesis, non-vector-borne human transmission and animal reservoirs. Emerg Microbes Infect. 2016 Aug 24;5:e93. View Article
11. Evans SM, Kim K, Moore CE, Uddin MI, Capozzi ME, Craft JR, Sulikowski GA, Jayagopal A. Molecular probes for imaging of hypoxia in the retina. Bioconjug Chem. 2014 Nov 19;25(11):2030-7. doi: 10.1021/bc500400z. PubMed PMID: 25250692; PubMed Central PMCID: PMC4240343. View Article
12. Kenealey J, Subramanian P, Comitato A, Bullock J, Keehan L, Polato F, Hoover D, Marigo V, Becerra SP. Small Retinoprotective Peptides Reveal a Receptor-binding Region on Pigment Epithelium-derived Factor. J Biol Chem. 2015 Oct 16;290(42):25241-53. View Article
13. Uddin MI, Evans SM, Craft JR, Marnett LJ, Uddin MJ, Jayagopal A. Applications of azo-based probes for imaging retinal hypoxia. ACS Med Chem Lett. 2015 Feb 12;6(4):445-9. View Article
14. Asnaghi L, Tripathy A, Yang Q, Kaur H, Hanaford A, Yu W, Eberhart CG. Targeting Notch signaling as a novel therapy for retinoblastoma. Oncotarget. 2016 Sep 20. View Article
15. Taylor-Walker G, Lynn SA, Keeling E, Munday R, Johnston DA, Page A, Scott JA, Goverdhan S, Lotery AJ, Ratnayaka JA. The Alzheimer's-related amyloid beta peptide is internalised by R28 neuroretinal cells and disrupts the microtubule associated protein 2 (MAP-2). Exp Eye Res. 2016 Dec;153:110-121. View Article
16. Winokur PN, Subramanian P, Bullock JL, Arocas V, Becerra SP. Comparison of two neurotrophic serpins reveals a small fragment with cell survival activity. Mol Vis. 2017 Jul 3;23:372-384. eCollection 2017. View Article
17. Davis BM, Pahlitzsch M, Guo L, Balendra S, Shah P, Ravindran N, Malaguarnera G, Sisa C, Shamsher E, Hamze H, Noor A, Sornsute A, Somavarapu S, Cordeiro MF. Topical Curcumin Nanocarriers are Neuroprotective in Eye Disease. Sci Rep. 2018 Jul 23;8(1):11066. View Article
18. Mathew B, Ravindran S, Liu X, Torres L, Chennakesavalu M, Huang CC, Feng L, Zelka R, Lopez J, Sharma M, Roth S. Mesenchymal stem cell-derived extracellular vesicles and retinal ischemia-reperfusion. Biomaterials. 2019 Jan 9;197:146-160. View Article
19. Arranz-Romera A, Davis BM, Bravo-Osuna I, Esteban-Pérez S, Molina-Martínez IT, Shamsher E, Ravindran N, Guo L, Cordeiro MF, Herrero-Vanrell R. Simultaneous co-delivery of neuroprotective drugs from multi loaded PLGA microspheres for the treatment of glaucoma. J Control Release. 2019 Mar 10;297:26-38. View Article
20. Núñez-Álvarez C, Osborne NN. Blue light exacerbates and red light counteracts negative insults to retinal ganglion cells in situ and R28 cells in vitro. Neurochem Int. 2019 May;125:187-196. View Article
21. Chang C, Hu Y, Hogan SL, et al. Pharmacogenomic Variants May Influence the Urinary Excretion of Novel Kidney Injury Biomarkers in Patients Receiving Cisplatin. Int J Mol Sci. 2017;18(7):1333. Published 2017 Jun 22. View Article
22. Bullock J, Pagan-Mercado G, Becerra SP. Cell-based assays to identify novel retinoprotective agents. MethodsX. 2020 Aug 10;7:101026. View Article
23. Kim HM, Kwon J, Lee K, Lee JW, Jang DS, Kwon HC. Constituents of Gastrodia elata and Their Neuroprotective Effects in HT22 Hippocampal Neuronal, R28 Retinal Cells, and BV2 Microglial Cells. Plants (Basel). 2020 Aug 18;9(8):1051.  View article
24. Yadav M, Schiavone N, Guzman-Aranguez A, Giansanti F, Papucci L, Perez de Lara MJ, Singh M, Kaur IP. Atorvastatin-loaded solid lipid nanoparticles as eye drops: proposed treatment option for age-related macular degeneration (AMD). Drug Deliv Transl Res. 2020 Aug;10(4):919-944. View article
25. Yan B, Gao L, Huang Y, Wang X, Lang X, Yan F, Meng B, Sun X, Li G, Wang Y. Exosomes derived from BDNF-expressing 293T attenuate ischemic retinal injury in vitro and in vivo. Aging (Albany NY). 2020 Nov 29;12. View article 

If you publish research with this product, please let us know so we can cite your paper.