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Product Name | Monkey IFN-gamma/TNF-alpha/IL-2 FluoroSpot Kit |
Description | The FluoroSpot Plus assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The monkey IFN-γ/TNF-α/IL-2 FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. The included anti-CD3 mAb can be used to induce cytokine secretion and is dependent on number, nature and viability of accessory cells. The antibody cross-reacts with CD3 on cynomolgus and rhesus macaque T cells. The monoclonal antibodies in this kit react with human IFN-γ, TNF-α, and IL-2 and cross-react with IFN-γ, TNF-α, and IL-2 from non-human primates. |
Size | 2 plates |
Concentration | n/a |
Applications | FluoroSpot |
Other Names | interferon/interleukin EIA, assay, enzyme immunoassay, test |
Gene, Accession, CAS # | Gene ID: 3458, 7124, 3558 |
Catalog # | FSP-212822-2 |
Price | $1135 |
Order / More Info | Monkey IFN-gamma/TNF-alpha/IL-2 FluoroSpot Kit from MABTECH AB |
Product Specific References | PubMed: 27889562, 34686669, 28352649, 33588604 |