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Product Name | Recombinant human Ribonuclease A/RNASE 1 protein |
Description | Domain: 29-156aa. Recombinant human RNASE1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques. MW: 15.3kDa(134aa). Purity 90% by SDS - PAGE. N- terminal Sequence Analysis: Lys-Glu-Ser-Arg-Ala. RNASE1, also known as ribonuclease A, is a member of pancreatic ribonuclease enzyme family. It is a relatively small protein and is a basic protein (pI = 9.63). Also, It has four disulfide bonds in its native state. It cleaves specifically after pyrimidine nucleotides. Cleavage takes place in two steps: first, the 3',5'-phosphodiester bond is cleaved to generate a 2',3'-cyclic phosphodiester intermediate; second, the cyclic phosphodiester is hydrolyzed to a 3'-monophosphate. (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG) The highest activity is exhibited with single stranded RNA. It can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts |
Size | 10ug, 20ug, 50ug, 100ug, 250ug, 500ug, 1mg |
Concentration | 1mg/ml (determined by Absorbance at 280nm) |
Applications | SDS-PAGE, Enzyme Activity |
Other Names | RNASE1, ribonuclease A family member 1, pancreatic, RAC1, RIB1, RNS1, ribonuclease pancreatic, HP-RNase, RIB-1, RNase UpI-1, RNase 1, Ribonuclease A, RNase A |
Gene, Accession, CAS # | NCBI: NP_937878.1, UniProt: P07998 |
Catalog # | ATGP4051 |
Price | $140, $260, $350, $580, $1010, $1730, $2890 |
Order / More Info | Recombinant human Ribonuclease A/RNASE 1 protein from NKMAX Co. Ltd. |
Product Specific References | n/a |