RIPA Lysis Buffer from ROCKLAND IMMUNOCHEMICALS INC.
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| Product Name | RIPA Lysis Buffer |
| Description | RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and wash buffer for small-scale affinity pull-down applications, such as immunoprecipitation, since most antibodies and protein antigens are not adversely affected by the components of this buffer. In addition, RIPA Lysis Buffer minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. The following RIPA Lysis Buffer components have the following effects: Tris-HCl is a buffering agent prevents protein denaturation, NaCl is a salt that prevents non-specific protein aggregation, NP-40 is a non-ionic detergent to extract proteins; Na-deoxycholate and SDS are ionic detergents to extract proteins; and sodium azide is a bacteriostatic agent added to retard bacterial growth. RIPA Lysis Buffer is supplied as a ready-to-use solution that requires no preparation. We suggest that the user add protease and phosphatase inhibitors not included with this product prior to use. |
| Size | 50 mL |
| Concentration | 1X |
| Applications | Other, WB |
| Other Names | 1X RIPA Lysis Buffer, 1X RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer, RIPA Buffer |
| Gene, Accession, CAS # | n/a |
| Catalog # | MB-030-0050 |
| Price | $90 |
| Order / More Info | RIPA Lysis Buffer from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | PubMed: 20670848 // 25332149 // 26518752 // 28495207 // 29858079 // 31134659 // 31466866 // 32295130 // 34611843 // 34679020 // 34713936 // 34991629 // 35182754 // 35805580 |
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654
orders@rockland.com
Technical Support: tech@rockland.com
https://www.rockland.com/
Profile of ROCKLAND IMMUNOCHEMICALS INC.