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Antigenic Specificity | DDDDK-Tag |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | affinity purified |
Size | 50 µl, 100 µl, |
Concentration | > 0.5 mg/ml |
Applications | WB, IHC, IF/ICC, IP |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | DDDDK-Tag antibody is a Rabbit polyclonal antibody against DDDDK-Tag. Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A DDDDK can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects DDDDKged proteins. |
Immunogen | Fusion protein of human DDDDK. |
Other Names | DDDDK-Tag, DDDDK-Tag, DDDDK-Tag , DDDDK-Tag , DDDDK-Tag |
Gene, Accession # | n/a |
Catalog # | abx005577 |
Price | please inquire |
Order / More Info | DDDDK-Tag Antibody from ABBEXA LTD |
Product Specific References | n/a |
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