SMAD3 (phospho-S423) Antibody from BIORBYT LTD.
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| Antigenic Specificity | SMAD3 (phospho-S423) |
| Clone | polyclonal |
| Host Species | Rabbit |
| Reactive Species | human |
| Isotype | n/a |
| Format | immunoaffinity purified |
| Size | 25 ul |
| Concentration | 1.16 mg/mL |
| Applications | ELISA, FC, IHC, WB |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known. |
| Immunogen | Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human SMAD3 protein. |
| Other Names | rabbit SMAD3 pS423pS425, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2 |
| Gene, Accession # | UniProt: P84022 |
| Catalog # | orb345537 |
| Price | $292 |
| Order / More Info | SMAD3 (phospho-S423) Antibody from BIORBYT LTD. |
| Product Specific References | n/a |
BIORBYT LTD.
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