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Antigenic Specificity | Cytochrome C |
Clone | rCYCS/1010 |
Host Species | Recombinant Mouse |
Reactive Species | human, mouse, rat |
Isotype | IgG1, kappa |
Format | Protein G purified |
Size | 100 ug, 20 ug |
Concentration | n/a |
Applications | IHC-P, WB |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | In mammalian cells, the highly conserved cytochrome C protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached). |
Immunogen | A human partial protein corresponding to amino acids 81-104 of pigeon Cytochrome C was used as the immunogen for this recombinant Cytochrome C antibody. The epitope has been localized to amino acids 93-104. |
Other Names | n/a |
Gene, Accession # | UniProt: P99999 |
Catalog # | orb640148 |
Price | $764 |
Order / More Info | Cytochrome C Antibody from BIORBYT LTD. |
Product Specific References | n/a |
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