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Antigenic Specificity | FITC |
Clone | NI 239 |
Host Species | Mouse |
Reactive Species | n/a |
Isotype | IgG1 |
Format | unconjugated |
Size | 0.5 mg |
Concentration | n/a |
Applications | n/a |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Monoclonal mouse antibody to fluorescein isothiocyanate isomer 1. To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. |
Immunogen | n/a |
Other Names | n/a |
Gene, Accession # | n/a |
Catalog # | orb21166 |
Price | $512 |
Order / More Info | FITC Antibody from BIORBYT LTD. |
Product Specific References | n/a |
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