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Antigenic Specificity | TNF |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, primate |
Isotype | n/a |
Format | serum |
Size | 1 ml |
Concentration | 75 mg/mL |
Applications | ELISA, IF, IHC, WB |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antiserum has been heated to 56°C for 30 minutes. The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. |
Immunogen | The whole rabbit serum was prepared by repeated immunizations with recombinant human TNFa produced in E.coli. |
Other Names | APC1, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, MCF, Necrosin, Tumour Necrosis Factor Alpha, rabbit Tumor Necrosis Factor Alpha, rabbit TNF Alpha, Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2 |
Gene, Accession # | UniProt: P01375 |
Catalog # | orb750675 |
Price | $1805 |
Order / More Info | TNF Antibody from BIORBYT LTD. |
Product Specific References | n/a |
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