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Antigenic Specificity | Flamingo (starry night) |
Clone | Flamingo #74 |
Host Species | Mouse |
Reactive Species | Drosophila |
Isotype | IgG1 |
Format | supernatant |
Size | 1 ml |
Concentration | n/a |
Applications | Immunofluorescence,Immunohistochemistry,Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Epitope: Extracellular domain, aa 539-1219. Flamingo #74 does not work in IP. Another mouse monoclonal antibody to Flamingo #71 is appropriate for IP experiments. About requests of #71, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). In immunostaining pupal wings, we fix tissues with 4% paraformaldehyde (or 3.7% formaldehyde) in PBS on ice overnight (at least for 2 hours), and incubate tissues in the mAb diluted 1:10 in PBT containing 2% BSA. We do not prefer any fix solutions including EDTA or EGTA. This is because classic cadherins are degraded under Ca++- free conditions, although we have not studied how unstable Fmi is under that condition. A portion of the Fmi ectodomain (amino acid residues 539-1219) was used as immunogen. Flamingo is the protocadherin-like wing polarity protein stan. Antibody Registry ID: AB_2619583 |
Immunogen | Extracellular domain, aa 539-1219., Partial protein |
Other Names | CG11895, fmi, flam |
Gene, Accession # | stan, Gene ID: 36125, UniProt: Q9V5N8 |
Catalog # | Flamingo #74 |
Price | please inquire |
Order / More Info | Flamingo (starry night) Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK |
Product Specific References | PubMed: 1E+07 |