Flamingo (starry night) Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK

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Antigenic SpecificityFlamingo (starry night)
CloneFlamingo #74
Host SpeciesMouse
Reactive SpeciesDrosophila
IsotypeIgG1
Formatsupernatant
Size1 ml
Concentrationn/a
ApplicationsImmunofluorescence,Immunohistochemistry,Western Blot
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DescriptionEpitope: Extracellular domain, aa 539-1219. Flamingo #74 does not work in IP. Another mouse monoclonal antibody to Flamingo #71 is appropriate for IP experiments. About requests of #71, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). In immunostaining pupal wings, we fix tissues with 4% paraformaldehyde (or 3.7% formaldehyde) in PBS on ice overnight (at least for 2 hours), and incubate tissues in the mAb diluted 1:10 in PBT containing 2% BSA. We do not prefer any fix solutions including EDTA or EGTA. This is because classic cadherins are degraded under Ca++- free conditions, although we have not studied how unstable Fmi is under that condition. A portion of the Fmi ectodomain (amino acid residues 539-1219) was used as immunogen. Flamingo is the protocadherin-like wing polarity protein stan. Antibody Registry ID: AB_2619583
ImmunogenExtracellular domain, aa 539-1219., Partial protein
Other NamesCG11895, fmi, flam
Gene, Accession #stan, Gene ID: 36125, UniProt: Q9V5N8
Catalog #Flamingo #74
Priceplease inquire
Order / More InfoFlamingo (starry night) Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK
Product Specific ReferencesPubMed: 1E+07
DEVELOPMENTAL STUDIES HYBRIDOMA BANK
DEVELOPMENTAL STUDIES HYBRIDOMA BANK
DEVELOPMENTAL STUDIES HYBRIDOMA BANK
Dr. David Soll
University of Iowa Dept. Biology
Iowa City IA 52242
P: 319-335-3826
F: 319-335-2077

dshb@uiowa.edu

http://dshb.biology.uiowa.edu

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