Mouse 4-1BBL Antibody from ICHORBIO
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| Antigenic Specificity | Mouse 4-1BBL |
| Clone | TKS-1 |
| Host Species | Rat |
| Reactive Species | mouse |
| Isotype | IgG2a kappa |
| Format | Protein A/G purified, low endotoxin |
| Size | 25mg |
| Concentration | ≥ 5.0 mg/ml |
| Applications | Blocking, Functional Assays, Western Blot. RUO |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | Specificity: TKS-1 activity is directed against 4-1BBL (CD137L). 4-1BBL (CD137L) and 4-1BB (CD137) are a receptor ligand pair belonging to the tumor necrosis factor (TNF) receptor/TNF superfamily. 4-1BBL is a type II transmembrane protein expressed by activated B cells, macrophages, and dendritic cells. Members of the TNF-TNF receptor superfamily play critical roles in regulating cellular activation, differentiation, and apoptosis and the 4-1BBL-4-1BB interaction is important in cellular immune responses. 4-1BBL-4-1BB interaction provides a co-stimulatory signal to T cells, resulting in increased proliferation and cytokine production.A TKS-1-producing hybridoma was generated by immunizing a SD rat with 4-1BBL-transfected NRK cells (rat IgG2a,κ). Splenocytes were fused with P3U1 cells and hypoxanthine-aminopterin-thymidine selection was performed. The monoclonal antibody was identified by its strong reactivity with 4-1BBL-transfected L5178Y cells, cloned by limiting diffusion, purified from ascites, and found to bind to 4-1BBL/NRK and 4-1BBL/P815 cells.Pre-incubation with TKS-1 blocks 4-1BB-Ig binding to 4-1BBL/L5178Y cells, indicating TKS-1 is specific to mouse 4-1BBL and can interrupt the interaction between 4-1BBL and 4-1BB. Additionally, TKS-1 can block anti-CD3-stimulated T cell proliferation co-stimulated by 4-1BBL /P815 cells. The monoclonal antibodies 19H3 and TKS-1 bind to different sites on murine 4-1BBL, with TKS-1 binding to a site that overlaps with the receptor binding site. Additionally, TKS-1 and 4-1BB bind to a similar site, making TKS-1 especially useful for blocking studies. |
| Immunogen | BALB/c mouse B lymphoma line 2PK-3 |
| Other Names | CD137L, TNFSF9, 4-1BB Ligand, TKS-1 |
| Gene, Accession # | n/a |
| Catalog # | ICH1184-25mg |
| Price | $1100 |
| Order / More Info | Mouse 4-1BBL Antibody from ICHORBIO |
| Product Specific References | n/a |
ICHORBIO
ICHORBIO
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