C-FOS Antibody from IMMUNOSTAR INC.

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Antigenic SpecificityC-FOS
Clonepolyclonal
Host SpeciesRabbit
Reactive Speciesmouse, rat
Isotypen/a
Formatserum
Size100 µl
Concentrationn/a
ApplicationsImmunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot
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DescriptionThe C-FOS Antibody was raised to synthetic peptide sequence corresponding to human c-fos (4-17) coupled to bovine thyroglobulin with glutaraldehyde. For induction of c-fos protein activity rats were injected with 1.0 ml of 1.5 M NaCl per 100 grams of body weight. Negative control rats were injected with the same volume of normal saline. The ImmunoStar c-fos antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat paraventricular nucleus and supraoptic nucleus using indirect immunofluorescent and biotin/avidin-HRP techniques. No labeling was seen in negative control rats. Recommended primary dilutions for these methods are 1/200-1/400 in PBS/0.3% Triton X-100 - FITC Technique and 1/4000-1/6000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Specificity of the antiserum was demonstrated by blockage of staining in experimental rats by omission of c-fos antibody or by substitution of antibody pre-incubated with synthetic peptide or the conjugate. Immunoblot analysis of mediobasal hypothalamus showed a single band of approximately 55-60 kD.
ImmunogenSynthetic human c-fos (4-17)
Other Namesn/a
Gene, Accession #n/a
Catalog #26209
Price$425
Order / More InfoC-FOS Antibody from IMMUNOSTAR INC.
Product Specific ReferencesPubMed: 23262236, 23085313, 21933288, 20214438, 16533665, 15219664, 12676287, 12353219, 10362318
IMMUNOSTAR INC.
IMMUNOSTAR INC.
IMMUNOSTAR INC.
P.O. Box 488
Hudson WI 54016-0488
P: 715-386-8407
P: 866-386-3500
F: 866-386-4500

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