Reactive Species | bird, bat, buffalo, bullfrog, canine, cat, chick, chicken, dove, fish , fowl, fox, frog, goldfish, guinea pig, hamster, hedgehog, hen, human, lizard, mink, mole, mollusk, monkey, mouse, mudpuppy, sheep, porcine, pigeon, platypus, possum, rabbit, raccoon, sheep, rat, Scorpaena porcus (fish), sea lamprey, sheep, shrew, snake, sparrow, Sparus aurata (fish), squirrel, starfish, sting ray, suncus murinus (shrew), tadpole, turkey, turtle, worm (spionidae), zebra finch (bird), zebrafish |
Description | The antibody has a proven strong fluorescent staining at a 1/200-1/400 dilution and a strong Biotin-Streptavidin/HRP staining at a 1/4000-1/6000 dilution in rat amygdala, cortex, and suprachiasmatic nucleus. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-5 M. VIP immunolabeling was completely abolished by pre-adsorption with VIP. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: Secretin, gastric inhibitory polypeptide, somatostatin, glucagon, insulin, ACTH, gastrin 34, FMRF-amide, rat GHRF, human GHRF, peptide histidine isoleucine 27, rat pancreatic polypeptide, motilin, peptide YY, substance P, neuropeptide Y, and CGRP. The histochemical antibody for VIP is generated in a rabbit against porcine VIP conjugated to bovine thyroglobulin with carbodiimide. The antibody is provided as 100 µL of lyophilized whole serum, and 0.09% sodium azide. |
Product Specific References | PubMed: 1348629, 1317556, 1283961, 1282372, 24469635, 24725136, 25071465, 25540807, 25932952, 25988808 |