Connexin-32 Antibody from KERAFAST, INC.

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Antigenic SpecificityConnexin-32
CloneM12.13
Host SpeciesMouse
Reactive Speciesmouse, rat, human
IsotypeIgG
Formatunconjugated
Size100ug
Concentrationn/a
ApplicationsIHC (acetone or unfixed tissues recommended. Epitope is internal, paraffin sections are untested. Works with lightly formaldehyde-fixed tissue). WB (Detects a 27 kDa Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47 kDa band corresponding to aggregates).
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DescriptionAnti-Connexin-32 [M12.13] Antibody. Target: Connexin-32, Gap junction beta-1 protein, Cx32, Gjb1. Epitope: On cytoplasmic surface, likely between residues 95-125
Immunogenisolated rat hepatocyte gap junctions
Other Namesn/a
Gene, Accession #Accession: P08034
Catalog #EF0013
Price$336
Order / More InfoConnexin-32 Antibody from KERAFAST, INC.
Product Specific References
  1. Goodenough DA, Paul DL, Jesaitis L. 1988. Topological distribution of two Connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions. J.Cell Biol. 107:1817-1824.
  2. Hamzei-Sichani F, Davidson KG, Yasumura T, Janssen WG, Wearne SL, Hof PR, Traub RD, Gutierrez R, Ottersen OP, Rash JE. Mixed Electrical-Chemical Synapses in Adult Rat Hippocampus are Primarily Glutamatergic and Coupled by Connexin-36. Front Neuroanat. 2012 May 15;6:13.
  3. Rela L, Bordey A, Greer CA. Olfactory ensheathing cell membrane properties are shaped by connectivity. Glia. 2010 Apr 15;58(6):665-78. doi: 10.1002/glia.20953.
  4. Fowler SL, McLean AC, Bennett SA. Tissue-specific cross-reactivity of connexin32 antibodies: problems and solutions unique to the central nervous system. Cell Commun Adhes. 2009 Dec;16(5-6):117-30.
  5. Rash JE, Olson CO, Pouliot WA, Davidson KG, Yasumura T, Furman CS, Royer S, Kamasawa N, Nagy JI, Dudek FE. Connexin36 vs. connexin32, "miniature" neuronal gap junctions, and limited electrotonic coupling in rodent suprachiasmatic nucleus. Neuroscience. 2007 Oct 26;149(2):350-71. Epub 2007 Jul 21.
  6. Rash JE, Olson CO, Davidson KG, Yasumura T, Kamasawa N, Nagy JI. Identification of connexin36 in gap junctions between neurons in rodent locus coeruleus. Neuroscience. 2007 Jul 29;147(4):938-56. Epub 2007 Jul 2.
  7. Fischer R, Reinehr R, Lu TP, Schonicke A, Warskulat U, Dienes HP, Haussinger D. Intercellular communication via gap junctions in activated rat hepatic stellate cells. Gastroenterology. 2005 Feb;128(2):433-48.
  8. Rash JE, Pereda A, Kamasawa N, Furman CS, Yasumura T, Davidson KG, Dudek FE, Olson C, Li X, Nagy JI. High-resolution proteomic mapping in the vertebrate central nervous system: close proximity of connexin35 to NMDA glutamate receptor clusters and co-localization of connexin36 with immunoreactivity for zonula occludens protein-1 (ZO-1). J Neurocytol. 2004 Jan;33(1):131-51.
  9. Alvarez-Maubecin V, Garcia-Hernandez F, Williams JT, Van Bockstaele EJ. Functional coupling between neurons and glia. J Neurosci. 2000 Jun 1;20(11):4091-8.
  10. Day WE, Bowen JA, Barhoumi R, Bazer FW, Burghardt RC. Endometrial connexin expression in the mare and pig: evidence for the suppression of cell-cell communication in uterine luminal epithelium. Anat Rec. 1998 Jul;251(3):277-85.
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