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Antigenic Specificity | CD8b, Human |
Clone | REA715 |
Host Species | Recombinant Human |
Reactive Species | human |
Isotype | IgG1 |
Format | biotin conjugate |
Size | 100 tests in 200 µL |
Concentration | 1:50 |
Applications | Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | CD8b Antibody, anti-human, Biotin, REAfinity™. Clone REA715 recognizes the human CD8b antigen, a single-pass type I membrane protein and member of the immunoglobulin superfamily, also known as T cell surface glycoprotein Lyt-3. The CD8 glycoprotein is expressed by thymocytes, mature T cells, and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex class I antigens and in signal transduction during the course of T cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. Most of peripheral blood CD8+ T lymphocytes express the CD8a/CD8b heterodimer, while CD8+CD16+ natural killer cells and CD8+ T cell receptor γ/δ+ T lymphocytes express only the CD8a/CD8a homodimer. CD8b can therefore be used to selectively bind to CD8+ T cells while excluding CD8+ NK cells. | Additional information: Clone REA715 displays negligible binding to Fc receptors. |
Immunogen | n/a |
Other Names | CD8 beta chain, CD8 b chain, CD8b |
Gene, Accession # | Gene ID: 926 |
Catalog # | 130-110-508 |
Price | $264 |
Order / More Info | CD8b, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | Norment, A. M. et al. (1988) A second subunit of CD8 is expressed in human T cells. EMBO J. 7 (11): 3433-3439. | Baume, D. M. et al. (1990) Differential expression of CD8 alpha and CD8 beta associated with MHC-restricted and non-MHC-restricted cytolytic effector cells. Cell. Immunol. 131 (2): 352-365. | Terry, L. A. et al. (1990) Differential expression and regulation of the human CD8 alpha and CD8 beta chains. Tissue Antigens 35 (2): 82-91. |