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Antigenic Specificity | IL-10, Human |
Clone | JES3-9D7 |
Host Species | Rat |
Reactive Species | human, nonhuman primate |
Isotype | IgG1κ |
Format | allophycocyanin (APC) conjugate |
Size | 30 tests in 60 µL |
Concentration | 1:50 |
Applications | Intracellular flow cytometry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | IL-10 Antibody, anti-human, APC. Interleukin 10 (IL?10) is a cytokine predominantly secreted by CD4+ memory and effector T cells and antigen-presenting cells, for example, monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by T?1 cells, activated monocytes, and NK cells. It can stimulate immunoglobulin production by B cells. | The Anti-IL?10 antibodies has been designed for intracellular staining of IL?10-producing cells. Cells can be stimulated for IL?10 production, for example, by polyclonal stimulation with mitogens. For induction of IL?10 production by antigen-specific T cells, cells are re-stimulated with the respective antigen. IL?10 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL?10-producing cells can be stained intracellularly with Anti-IL?10 antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL?10-producing cells. | Magnetically enriched cells can be stained intracellularly for IL?10 production directly on the MACS® Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures. The protocol is useful for cytokine analysis of rare cells, for example, CD4+ T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages or synovial fluids. |
Immunogen | n/a |
Other Names | IL10, CSIF, GVHDS, TGIF, IL10A |
Gene, Accession # | Gene ID: 3586 |
Catalog # | 130-117-824 |
Price | $136 |
Order / More Info | IL-10, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | n/a |