Edit |   |
---|---|
Antigenic Specificity | MPO, Human |
Clone | REA491 |
Host Species | Recombinant Human |
Reactive Species | human |
Isotype | IgG1 |
Format | phycoerythrin (PE) conjugate |
Size | 30 tests in 60 µL |
Concentration | 1:50 |
Applications | Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Immunocytochemistry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | MPO Antibody, anti-human, PE, REAfinity™. Clone REA491 recognizes the human myeloperoxidase (MPO) antigen, a heterotetrameric protein consisting of two 15 kDa light chains and two variable-weight glycosylated heavy chains bound to a prosthetic heme group. MPO produces hypochlorous acid from hydrogen peroxide and chloride anion during the neutrophil's respiratory burst. Furthermore, it oxidizes tyrosine to tyrosyl radical using hydrogen peroxide as an oxidizing agent. MPO is present in azurophilic granules of neutrophilic granulocytes and monocytes and is also expressed in some acute myeloid leukemia cells. It plays a major role in the oxygen-dependent microbicidal system of of polymorphonuclear leukocytes. The deficiency of this enzyme activity results in a marked microbicidal defect. | Additional information: Clone REA491 displays negligible binding to Fc receptors. |
Immunogen | n/a |
Other Names | Myeloperoxidase |
Gene, Accession # | Gene ID: 4353 |
Catalog # | 130-120-148 |
Price | $119 |
Order / More Info | MPO, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | Adair, J. E. et al. (2018) Novel lineage depletion preserves autologous blood stem cells for gene therapy of Fanconi anemia complementation group A. Haematologica 103 (11): 1806-1814. | Manivannan, P. et al. (2014) Can threshold for MPO by flow cytometry be reduced in classifying acute leukaemia? A comparison of flow cytometric and cytochemical myeloperoxidase using different flow cytometric cut-offs. Haematologica: Epub ahead of print. | Morishita, K. et al. (1987) Molecular cloning and characterization of cDNA for human myeloperoxidase. J. Biol. Chem. 262 (8): 3844-3851. | Papayannopoulos, V. et al. (2010) Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps. J. Cell Biol. 191 (3): 677-697. |