IL-10, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG
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| Antigenic Specificity | IL-10, Human |
| Clone | JES3-9D7 |
| Host Species | Rat |
| Reactive Species | human, nonhuman primate |
| Isotype | IgG1κ |
| Format | Vio B515 conjugate |
| Size | 100 tests in 1 mL |
| Concentration | 1:10 |
| Applications | Intracellular flow cytometry |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | IL-10 Antibody, anti-human, Vio® B515. Interleukin 10 (IL?10) is a cytokine predominantly secreted by CD4+ memory and effector T cells and antigen-presenting cells, for example, monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by T?1 cells, activated monocytes, and NK cells. It can stimulate immunoglobulin production by B cells. | The Anti-IL?10 antibodies has been designed for intracellular staining of IL?10-producing cells. Cells can be stimulated for IL?10 production, for example, by polyclonal stimulation with mitogens. For induction of IL?10 production by antigen-specific T cells, cells are re-stimulated with the respective antigen. IL?10 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL?10-producing cells can be stained intracellularly with Anti-IL?10 antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL?10-producing cells. | Magnetically enriched cells can be stained intracellularly for IL?10 production directly on the MACS® Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures. The protocol is useful for cytokine analysis of rare cells, for example, CD4+ T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages or synovial fluids. |
| Immunogen | n/a |
| Other Names | IL10, CSIF, GVHDS, TGIF, IL10A |
| Gene, Accession # | Gene ID: 3586 |
| Catalog # | 130-108-100 |
| Price | $370 |
| Order / More Info | IL-10, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
| Product Specific References | n/a |
MILTENYI BIOTEC B.V. & Co. KG
MILTENYI BIOTEC B.V. & Co. KG
Friedrich-Ebert-StraÃe 68
51429 Bergisch Gladbach GERMANY
P: +49 2204 8306-0
F: +49 2204 85197
macs@miltenyibiotec.de
https://www.miltenyibiotec.com
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